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Image scaling

Use the Image Scaling window to define Z scaling for the image.

  1. Enter the distance between images.
  2. Click the Update image file button.
  3. In the Update Image File Scaling window, check Allow scaling to be written to image file and click OK.
  4. In the Image Scaling window, click OK.
  5. Save as a .jpx file (recommended) or use the original file format.

Z scaling applied to images settings

ClosedDistance between images

Use the focal distance or the physical distance.

ClosedFocal Distance

Image stacks collected with the program are collected using spacings that describe the focal plane separation.

ClosedPhysical Distance

Describes the physical movement of the microscope stage as images are collected.
If you select this option, you need to select the correction factor for the physical distance between the lens and the image. The program automatically enter this value for Air, Oil, and Water. If you select Other, you must manually enter the factor.

ClosedUpdate image file

Use this button to save the scaling information with the image file (it is already automatically saved with the data file).