Calibration overview

A properly calibrated system is essential for successful and efficient software use. Follow our guidelines carefully to ensure proper system function (PDF).

 

Equipment

• A microscope system (camera, stage, objectives, computer etc.)
• Calibration grid slide
• The latest software version
• Optional: Allen wrenches for aligning camera and Stage.

Before you start...

1. Turn on the microscope system and the computer.
2. Start the program.
3. Load the calibration grid slide onto the stage.

Overview of the calibration process

1. Add and tighten the objectives:
   • Tighten all the objectives by hand to make sure they have not come loose.
   • Add any new objectives to the microscope BEFORE calibrating the system.
2. Verify that camera and stage are aligned.
3. Calibrate lenses with calibration grids.
4. Perform parcentric/parfocal calibration.
5. Check the accuracy of the calibration.

 

Consult the Troubleshooting section if necessary.

 

Also seeCreating a lens, Creating a new lens based on a calibration grid slide , Editing a lens