Deconvolution overview
Using the Huygens algorithm, the Deconvolution module reverses the optical distortion that takes place in an optical microscope to create clearer images. It can sharpen images that are affected by fast motion or jiggles during acquisition, or images that have some type of noise introduced into the signal.
How can I improve the deconvolution process?
Step sizeHow do I use deconvolution?
All images should be closed before you start. Parameters are described below the slideshow.
Tell me more about the Deconvolution Parameters
Deconvolution doesn't correct highly auto-fluorescent background signal (tissue, culture media, etc.) because the deconvolution algorithms interpret the background as a real signal (i.e., as similar to all the other light intensities collected). As a result, the background is deconvolved along with the relevant experimental signal.