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Acquire Multichannel Image

Use to acquire fluorescent and false colored images.

 

  1. Live image mode is on.
  2. The device command sequences necessary for each channel are defined.
  3. Configure your system for a multichannel acquisition. Automating the Multichannel Acquisition Process
  4. Click Acquisition>Video Tool Panels>Show All Video Tools to open the three tool panels used during the acquisition.
  5. The Multichannel Control tool panel displays the channels configured in Step 2 .

    6. Optional : Click the Setup button to review the channel's settings.

    1. Check the Remember Video Settings checkbox.
    2. Click the device command sequence button for the first channel; adjust the exposure in the camera settings until a full balanced histogram is achieved.
    3. Click the device command sequence for the next channel; adjust the exposure in the camera settings until a full balanced histogram is achieved.
    4. Click the device command sequence for the last channel; adjust the exposure in the camera settings until a full balanced histogram is achieved.
    5. Neurolucida has "memorized" the camera settings for each channel and is ready to apply them for acquisition.
  6. Click Acquisition>Acquire Multichannel Image.
  7. Neurolucida performs the acquisition tasks defined for the first channel.
  8. Neurolucida may prompt you to adjust the camera settings. Click OK.
  9. Neurolucida performs the acquisition tasks for the other channels defined.

 

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