Closed surface analysis

Purpose

Use this analysis to measure or count structures or portions of structures within a 3D structure generated by adding a skin to closed contours that are aligned in the Z direction.

Procedure

1. Select the structures you want to analyze.

2. Click to access closed surface analysis for neuronal trees or puncta as follows:

   • Axons and dendrites: Go to Analyze > Neuron data > Anatomical > Closed surface analysis

   • Puncta: Go to Analyze > Region data > Punctum > Closed Surface > Puncta in closed surface

In the example below, an axon enters and exits the 3D structure four times. In the "length" column, you can see the length of each part of the axon that is contained within the 3D structure.