Use to determine a cell's location based on multiple markers used as a result of double labeling.
Double labeling of cells occurs when cells are imaged using two or more imaging techniques, and marked more than once. Using several imaging techniques make it possible to visualize different aspects of the cells, but depending on the imaging technique, cells may appear to be in slightly different locations.
Multiple markers are considered to be the same cell (i.e, they co-localize) if they are in close proximity, that is, if a sphere of a given radius placed at one marker encloses another marker.
The size of the radius should be:
If the radius is set too large, distinct cells may be interpreted as a single cell.
The report displays the number of times a marker occurs within the designated radius of another marker.
The results are not listed twice: if Marker
1 and Marker 2 co-localize, the number of co-localizations is only listed for Marker 1.
Neurolucida Explorer 11 | MBF Science Support Center | Downloads