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Spaceballs step 2: Set microscope to low magnification

1. Switch to a low power objective on your microscope, such as 2x or 4x.
2. Select the matching lens from the Select Low Mag Lens drop-down menu.

The objective should:

• Show a substantial portion of your region of interest (ROI) in the field of view.
• Be suitable for tracing a contour around your ROI.

If it is difficult to distinguish the anatomical borders of your ROI at a low magnification, use a higher magnification for tracing.

Trace region(s) of interest

See Spaceballs Workflow

Microlucida 3 | MBF Bioscience Support Center | Downloads