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Maximum / Minimum Intensity Projection

To visualize an entire image stack in a single plane, use the Maximum Intensity Projection with light-on-dark images (e.g., fluorescence) or Minimum Intensity Projection with dark-on-light images (e.g., brightfield). See the Deep Focus method for an alternative.

When you select Image>Maximum (Minimum) Intensity Projection,Microlucidadisplays the projection.

To view the original image stack, go to Image>Maximum(Minimum) Intensity Projection and un-check to turn the projection off.
To save the projection image, click File>Image Stack> Image Stack Max (Min) Projection Save As.

How it works

Microlucida identifies the maximum (or the minimum) intensity value of each pixel along the z axis is projected onto the 2D plane.