Save As Current Plane

Purpose

Save only the current plane in an image stack—that is, the plane currently displayed in the main Microlucida window.

Note that this command is not available when a single-plane (2D) image is the current image.

Procedure and options for saving your file

  1. Bring the image plane that you want to capture into view.

  2. Go to File > Save As > Image Stack and click the Current Plane button.

  3. You'll see a warning that only a single image plane in an image stack will be saved.

    Click OK to proceed. You can check the box Suppress this message to prevent the warning from appearing in the future.

  4. In the dialog box that opens, choose options and save or export the image stack as follows:

    1. Navigate to the desired location for the file and type in a File Name.

    2. (optional) Choose from the following options:

      • Save/Export as type drop-down menu:

        • MBF JPEG2000 is the default file type: we recommend it.

        • MBF JPEG2000 and MBF TIFF files can include high bit-depth pixel data (if present) and image-display adjustments you've made in Microlucida. Metadata about the image, such as the microscope hardware used, is also saved.

        • JPEG2000 file format offers smart compression that can result in a significant reduction in the amount of storage space needed, with minimal data loss.

        • TIFF file format is uncompressed.

      • Export the image file: Check the box to save (export) the image and break its link to any associated data file.

        When checked, the Save button changes to an Export button.

        • The original image-display information and any high bit depth data that were present are lost.

        • This enables selection of additional Image Detail options.

      • Image Detail options:

        • Save as individual channels [available for multiple color-channel images]: Saves each color-channel in the image as a separate file.

          The channel number is added to the end of the file name, e.g., "FileName_Ch01.jp2"

        • Save image color [available when you select Save as individual channels]: Saves the color information with each separate channel or stack.

      • Compression [available when either JPEG2000 file type is selected]: Use the slider to indicate the amount of file compression desired.

    3. Click the Save or Export button to save/export the file and close the dialog box.