Stage setup
The options available (and displayed in additional tabs) vary depending on the stage and/or focus controller selected.
Changing the stage controller and/or settings can damage the stage, microscope, or other equipment. If you think changes are needed to your stage setup, please contact MBF Bioscience Technical Services for assistance:
https://www.mbfbioscience.com/welcome-technical-services
Focus knobs on the microscope
Do not use the fine focus knob on the microscope if you have a focus motor, as this can strip the gears of the focus motor.
- It is possible to use the coarse focus on the microscope, but keep in mind that these movements in the Z-axis are not recorded by Microlucida unless your system has a focus-position encoder. This would be problematic for a 3D reconstruction or a multi-section neuron tracing since the Z-axis information is essential to the accuracy of your data.
- Unless you have an internal Z motor on your microscope, we recommend that you avoid using the focus knobs on the microscope after the initial set-up of the slide on the microscope.